Background : The incidence of breast cancer worldwide is still high. Surgery remains
the top choice with other modalities of chemotherapy. radiation.and immunotherapy
such as Artemisia vulgaris (AV). Purpose : The study was aimed to demonstrate that
administration of AV extract increased the levels of p53 and Caspase-8 in
adenocarcinoma mammae. M ethods : This study used "Post test only control group
design" on 24 females C3H mice that were randomly selected and divided into four
groups : group K (control). P1 (chemotherapy). P2 (extract). and P3 (combination).
Adenocarcinoma mammae comes from the inoculation of donor mice. Chemotherapy
of Adriamycin 0.18 mg and Cyclophosphamide 1.8 mg were given in 2 cycles. AV 13
mg (0.2 ml) was given once daily orally. P53 and Caspase-8 levels were evaluated by
imunohistochemical staining. Results : Mean of p53 and Caspase-8 levels were
found in groups K. P1. P2. P3 were 22.06+1.73. 37.16+1.20. 24.60+1.08. 39.78+1.19
dan 17.16+1.28. 26.20+1.11. 24.60+1.08. 39.78+1.19. The statistical analysis
showed that there were significant differences in the levels of p53 between groups of
K vs P1. P3 (p=0.001). K vs P2 (p=0.048). P1 vs P2 (p=0.001). P1 vs P3 (p=0.039). P2
vs P3 (p=0.001). and in Caspase-8 between groups of K vs P1. P3 (p=0.001). K vs P2
(p=0.048). P1 vs P2 (p=0.001). P1 vs P3 (p=0.039). P2 vs P3 (p=0.001). Correlation
analysis between p53 and Caspase-8 showed significant correlation (p=0.047 dan
r=0.883). Conclusion : Artemisia vulgaris can improve the effectivity of AdriamycinCyclophosphamide chemotherapy on C3H mice with adenocarcinoma mammae in
terms of elevated levels of P53 and Caspase-8.